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measured at 405 nm, the detection limit of the assay is 4 10 12 M alkaline phosphatase in the original serum sample. While this may seem like a very low concentration, diagnostic tests often require an improved detection limit. A cascade ampli cation method has been proposed for improving the detection limit of the alkaline phosphatase assay, and involves a different synthetic substrate (Eq. 3.21), (oxotri uorobutyl)phenylphosphate:11

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The difference between Hardware and Software RAID is where the disk management is performed. Hardware RAID functions are performed, as the name implies, within the disk hardware. The disk array has dedicated internal CPU, I/O, and memory, to perform these functions without impacting the host computer. Management of Hardware RAID is performed through an interface program that runs on the host computer; but any management work that is to be performed is done within the disk array. If you take another look back at Figure 7.2, you ll see that Hardware RAID performs its functions independently from the operating system and independently of any software-based disk management. The output of a Hardware RAID function is a LUN, which looks exactly like a disk to the host system,

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3:21 When this substrate is dephosphorylated, its product acts as an inhibitor of a second enzyme, rabbit liver esterase. Thus, as alkaline phosphatase activity increases, there is a corresponding increase in the concentration of inhibitor produced, and consequently a decrease in the activity of the esterase. A second synthetic substrate was prepared for the (relatively nonselective) esterase, so that the indicator reaction is as shown in Eq. 3.22 below:

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regardless of whether its underlying configuration is a 9-disk RAID-0 stripe, a 14-way RAID-1 mirrored set, a 6-way RAID-5 stripe with cache in front, or something else entirely In general, users need not be concerned with the RAID level that is used on a Hardware RAID array The assumption that data is being properly managed should be adequate for most situations Of course, your mileage may vary Some of the advantages of Hardware RAID are: Performance The performance of parity RAID is more acceptable in a Hardware RAID configuration than in a Software RAID configuration The CPU overhead is offloaded from the host system to the dedicated outboard CPU Operating system neutrality No special software is usually required on a host to connect to a Hardware RAID system, so arrays can be connected to just about any kind of system that knows how to speak to disks.

If you can connect a disk array to your Amiga or your Xbox, it should work with all of its native features intact Disk cache Many disk array vendors put cache memory in their arrays This cache will greatly improve write performance and can improve read performance when the data that is being searched for remains in the write cache Other enhancements, such as read-ahead, where the array prereads data from the disk it expects will be requested soon, will also generally improve read performance Putting disk cache in front of a slower parity RAID model usually counteracts the performance disadvantages they impose Advanced features Some Hardware RAID manufacturers offer features like wide area data replication, or the ability to share expensive disk hardware between multiple hosts There are also disadvantages to Hardware RAID Depending on the vendor you choose, some or all of these may be present.

3:22 The substrate for the indicator reaction, dichloroindophenylbutyrate, is pale yellow. The product of the reaction is deep blue, with an absorbance maximum at 620 nm. The ingenuity of this method lies in the production of an enzyme inhibitor, (oxotri uorobutyl)phenol, rather than a species that is directly quantitated. The inhibitor affects the activity of the esterase, resulting in catalytic ampli cation of the initial alkaline phosphatase activity. The detection limit of the ampli ed assay has been reported as 3:2 10 14 M, a 100-fold improvement over the detection limit of the standard alkaline phosphatase assay. 3.5.1.2. Fluorescence. The use of molecular uorescence spectroscopy for the quantitation of enzyme reaction products has resulted in detection limits that are several orders of magnitude lower than those achieved by standard absorbance methods. At low analyte concentrations, uorescence emission intensity is directly proportional to concentration, and its value depends on both the molar absorptivity of the analyte at the excitation wavelength, and the uorescence quantum yield of the analyte, under the assay conditions. Fluorescence methods are inherently capable of detecting much lower concentrations of analytes, because of the instrumental principles involved. Consider, for example, a comparison of the light intensities measured in blank solutions by

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