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linear increases observed with absorbance and uorescence measurements. Since photons are a product of the reaction, they are produced in stoichiometric proportion to the amount of substrate consumed. Absorbance and uorescence measurements, on the other hand, produce a steady stream of detectable photons. For this reason, light intensities are much lower with luminescence reactions, and signals are generally integrated over several minutes to yield acceptable S/N levels. Instrumental requirements for luminescence measurements are fairly simple: A standard luminescence photometer consists of a light detector (photon counter) surrounding a sample holder inside an enclosure that eliminates ambient light; neither light source nor monochromator are required. Primary reactions that produce hydrogen peroxide have been tested14 with a luminol peroxidase indicator reaction, Eq. 3.24:

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If you must do parity RAID, then it is probably best to do so with Hardware RAID Writing in software-based RAID-5 is generally too slow for most high-volume, insert- or update-intensive production environments When Software RAID-1 is used, the performance is generally fine for most applications..

9

7

3:24

The other downside worth mentioning is that in order to bring Software RAID to a new operating system, its code must be ported. This can delay the availability of such products on emerging operating systems or prevent their availability altogether.

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Software RAID is often just one feature of logical volume management (LVM) software. LVM offers many other features besides Software RAID. While each LVM product is different, here are some of the more useful features: Online reconfiguration. This feature includes the ability to add and remove mirrors, to expand and, in some cases, shrink a volume or filesystem, and rearrange the disk layout of data, and to do so without interrupting user service. Removal of OS-imposed limitations. For example, in Sun s Solaris operating system, a disk cannot be divided into more than seven slices. A good LVM would remove that restriction, allowing whatever slicing is desired. Hooks into system management tools. When the LVM detects failures or performance issues in monitored devices, the information should be automatically shared with system monitoring tools. The LVM might even offer suggestions on how to better layout data for improved performance. Hot sparing. If one disk fails, a dedicated spare disk can be automatically moved into its place, transparent to the users. Some commercial examples of LVMs are VERITAS Software s Volume Manager, which runs on Solaris, HP-UX, AIX, Windows, and Linux, and Sun s Solstice Disk Suite.

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The oxidized form of luminol is produced in the excited state, and the emitted light is integrated over 4 min. When this reaction was used as an indicator reaction for a glucose assay using glucose oxidase, linearity was observed between 0.1- and 2.0-mM glucose, while a cholesterol assay using cholesterol oxidase yielded a linear calibration curve between 50 and 100 mg/mL. The sensitivity of the indicator reaction, and thus of both of the assays, depended strongly on the particular peroxidase used. Arthromyces (bacterial) peroxidase was found to yield calibration curve slopes that were 28 times greater for the glucose assay and 134 times greater for the cholesterol assay than horseradish peroxidase. Fire y luciferase15 may be employed as an indicator reaction for any primary enzymatic reaction that produces ATP. Luciferase catalyzes the oxidative decarboxylation of luciferin according to Eq. 3.25:

When designing a system, subscribe to the physics definition of a gas as applied to disks: Disk usage will always expand to consume all available resources. Extra disk capacity, especially disks kept offline, are vital to stave off disaster. A full filesystem presents the same problems with nonavailability of data storage as a down system. If a user needs to save a file and cannot, then the system may as well be down. In fact, the inability to save critical work after it has been performed may be worse than the system being down. It is certainly more frustrating to the average user, due to the wasted time.

3:25 This reaction results in light emission between 540 and 600 nm. Luciferase is activated by divalent magnesium, so experiments are carried out in the presence of excess Mg2 (1 mM). This reaction does not yield a constant light output under pseudo- rst-order conditions, as shown in Figure 3.5, which is thought to be due to product inhibition of luciferase. Figure 3.5 also shows the effect of hydrophobic and amphipathic species on light output; these species may be quantitated by their inhibitory effect on the luciferase reaction.