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The electrons produced in this oxidation reaction result in a measured current that is directly proportional to H2O2 concentration. Amperometry has been used to quantitate inorganic phosphate (Pi)17 in a dualenzyme assay, shown in Eqs. 3.27 and 3.28: Phosphate Inosine Ribose-1-phosphate Hypoxanthine ! 3:27 Hypoxanthine 2 H2 O O2 ! Uric acid H2 O2
Storage Area Networks (SANs)
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4 or 40%, in the case of the generalized use of an application integration solution applied to the information systems of large enterprises, for one of: a number of applications greater than 150; a distribution of the applications over more than 50 machines; a number of dataflows to be processed daily in excess of 2,000 files or 100,000 messages modification; a degree of reuse of parameter settings of at least 20% In this case, the EGM is equal to: EGM = 04 + (06 *02 * (100 - 20)/100) = roughly 05 or 50%.
3:28
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Back in the Dark Ages of client/server storage (the mid-1980s), disks were connected directly to hosts through several different interconnects, the two most popular being Integrated Drive Electronics (IDE) and SCSI. For the amount of data being stored and transferred at the time, this was a perfectly sound way to connect the high-speed computers of the day to their high-speed data storage devices. Both technologies allowed several (up to seven for SCSI and up to two for IDE) disks to be connected to the same computer interface, while data flowed at around 5 to 10MB/ second. As computer systems grew faster and storage requirements increased, SCSI disks no longer physically fit inside their server cabinets, and so they were put in external storage cabinets. But then SCSI s cable length limitations became a problem. Performance also became a problem because all those disks had to contend with the limited bandwidth and processing speed of their host computers. Fibre Channel was applied to this problem in the early 1990s, giving storage interconnects much higher rates of performance, and increasing the allowable distance between system and storage from just a few meters up to 10 kilometers or more. Connections began to be made through a new protocol, called Fibre Channel Arbitrated Loop, which allowed more disks and more storage to be connected to an individual host. FC-AL was physically arranged in either the traditional loop structure, as shown in Figure 8.2, or with a hub in a star-shaped configuration that still functioned logically as a loop. Soon after, Fibre Channel switches
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started to come down in price (it was the high cost of these switches that forced early Fibre Channel configurations into hub-based loop configurations). Once switches achieved more market acceptance through lower prices, the loop became a fabric, and SAN storage could be shared between more hosts in more complex arrangements than ever before. This new architecture, depicted in the previous chapter in Figure 7.5, greatly increased the throughput and ease of management of the network. The difference between a hub and a switch is that a hub has a minimum of internal electronics; it merely takes a frame (essentially the same thing as a data packet in the SCSI world) and passes it on, with no processing being done to it along the way. A hub can only handle a single frame at a time, which can seriously limit the capacity of a SAN that depends on it. A switch creates virtual network routes between the devices that are communicating. A switch with 32 ports can have as many as 16 separate routes running through it simultaneously, as half of the ports are talking to the other half of the ports. Hubs are simple and relatively inexpensive; it is becoming fairly common to find Fibre Channel hubs being built into high-end RAID arrays. Switches remain independent boxes that are often mounted into a data center rack.
In addition to peroxide-producing reactions, amperometry may be used in conjunction with a variety of oxidase and dehydrogenase enzymes that employ low molecular weight mediators (Med) as electron acceptors in place of molecular oxygen. ! Sred n Med ox Pox n Med red
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