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magnitude of the alternating current response. This current is directly proportional to the conductivity of the solution, which, in turn, is dictated by ionic strength. The urease reaction, in particular, is ideally suited to conductimetric quantitation.18 Urea is hydrolyzed by urease according to Eq. 3.31: H2 NCONH2 3 H2 O 2 NH HCO OH ! 4 3

bonnie, we need to buy another terabyte so that we can have it for this host, clyde With a SAN, the unused terabyte can be reallocated, entirely through software, from bonnie to clyde When storage is physically (or at least logically; SANs do not require all the storage to be physically colocated for it to be centrally managed) centralized, it can be administered from a central console, by a small team of administrators, often just one administrator Centralized storage can also be backed up more easily to a centrally located set of SAN-capable tape drives or disks The result of both of these centralizations is a reduction in complexity (complexity is, of course, the sworn enemy of high availability) and in management costs, freeing up resources for other projects SAN management is often performed with the combination of a whiteboard in the system administrator s office and a spreadsheet.

Often, the information on the whiteboard and in the spreadsheet are not up-to-date, and sometimes they completely contradict each other Modern SAN management software eliminates the need to use these old-fashioned tools, replacing them with modern methods that can build and maintain a full-blown database, and that can discover new storage and new hosts as they are added to the SAN, simplifying management even further One problem that has plagued SANs in recent years is that there are so many different vendors, each offering different products and components, with different revision levels The result is a morass of combinations and permutations that ensure that virtually no two SANs have exactly the same combination of hardware, firmware, and software, and that nobody could possibly have tested all of the combinations In many cases, the combinations are not only untested, but they genuinely don t work.

3:31

The introduction of just one bad component can cause problems across the entire SAN in totally new and unexpected ways that can be extremely difficult to isolate The promise of SANs cannot be fully realized until the vendors can develop a basic set of standards that allow all of the different vendors to play together We sincerely hope that by the time you read these words most of these compatibility problems have been solved..

26 We presuppose here that we mostly have to supervise file dataflows. 27 In some large enterprises, thousands of person/days each year can be dedicated to tracking

Since there are many hosts connected to many storage devices in a SAN, the question invariably arises: Can we share data between the different hosts on the SAN The answer is a very qualified yes. The fundamental problem with two systems accessing the piece of data on the same disk at the same time is that standard operating systems and disk management software do not know how to arbitrate between these parallel requests, particularly if one of those requests is a write. The most benign outcome of such a conflict is probably a system crash from one or more of the hosts. Data corruption, potentially large-scale, is another potential, and far more serious, outcome.

In this reaction, four ions are produced from a single uncharged molecule of substrate, resulting in a signi cant increase in ionic strength. Good S/N may be achieved if the initial ionic strength (buffer concentration) of the assay medium is low, in the 5 10-mM range. 3.5.3. Other Detection Methods 3.5.3.1. Radiochemical. Radiochemical methods are increasingly becoming supplanted by methods that do not require specialized facilities, although in some cases, the method of choice still involves the use and quantitation of radioactive species. Examples include the endpoint assays for guanosine-50 -triphosphate (GTP) and guanosine-50 -diphosphate (GDP).19 These species are involved in hormone regulation, protein synthesis and secretion, and gluconeogenesis. The GTP assay involves Reactions 3.32 and 3.33:

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