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Figure 1.5. Relationship between actual and measured BSA concentration in six samples, showing that this method produces comparable results to the Bradford and BCA total protein assays. [Reprinted, with permission, from K. C. Bible, S. A. Boerner, and S. H. Kaufmann, Anal. Biochem. 267, 1999, 217 221. A One-Step Method for Protein Estimation in Biological Samples: Nitration of Tyrosine in Nitric Acid. Copyright # 1999 by Academic Press.]

Multiple conduits. Newer disks and networks often support the running of multiple, parallel cables between devices. While parallel cables can improve performance, the real gain is in availability. If a controller or HBA fails for one of the cables, data can continue to move between the devices using the second cable. If, however, the cables are run through the same conduits, and along the same paths between the two devices, the value in increased availability is limited. A single event could easily cut both cables, halting the flow of data. We offer a rather extreme policy to help reduce the cable overpopulation problem: If a cable is not labeled, it should be removed from the floor. Large data centers will simply giggle at this suggestion, and perhaps they should. But small data centers, shipboard data centers come to mind, may find this is an absolute requirement. They do not always have the 9 to 12 inches of space under their raised data center floors.

Although today s computer systems are less sensitive to overheating than systems from 10 and 20 years ago, it is still important to maintain reasonable

Figure 1.6. (a) Chemical structure of uorescamine, and (b) calibration curves obtained for different proteins. [Reprinted, with permission, from P. Bohlen, S. Stein, W. Dairman, and S. Undenfriend, Archives of Biochemistry and Biophysics 155, 1973, 213 220. Fluorometric Assay of Proteins in the Nanogram range. Copyright # 1973 by Academic Press, Inc.]

A significant number of consulting firms in the area of choosing application integration solutions offer typical questionnaires, enriched over the course of their successive experience. The reuse of these questionnaires can help accelerate the process of writing the RFP, and ensure that nothing important is forgotten. However, they are often too general for specific enterprise requirements. In addition, by using standard questionnaires originally from a particular consulting firm, it is not uncommon that providers already have the corresponding standard answers. How then can we be sure that the provider will understand the real need of the enterprise if that need is drowned in a questionnaire that is too rich in requirements and questions To be efficient, the RFP requirements and questions absolutely must be prioritized around the principal functions needed in the enterprise. Without this, it will not be possible to discern which service levels are essential and which are ultimately accessory. It is more worthwhile to select the questions that are important to the context of the enterprise, and put the emphasis on how the provider responds to those questions. 9.6.3. Key points in an RFP for application integration Let us recall that our objective is not to provide a complete treatise on finalizing IT specifications, but rather to indicate the points in the context of application integration that appear key to us. 9.6.3.1. Enterprise interest in an application integration solution Reminders: take account of general issues for the enterprise (departments, perspectives); recall how the implementation of an application integration solution is strategic for the enterprise. 9.6.3.2. Expression of need Recall the business objectives, both initial and target. Indicate the technical constraints, initial and target.

10

An older method employing uorescamine may be used if improved detection limits are required. In this method, uorescamine (Fig. 1.6) reacts with the primary amine groups of the protein, generating ourophores that can be excited at 390 nm to generate emission at 475 nm. Figure 1.6 also shows calibration curves for a variety of proteins; this method is capable of detecting as little as 10-ng total protein.10 Table 1.1 summarizes detection limits and interferences for the ve protein assay methods described here.