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Once when I was working in an OHF on a very hot August day in New York, I walked past one cage, where a system administrator sat, furiously typing on his keyboard. Nothing unusual about that, except that he was wearing a down ski parka, zipped up to his neck, a wool hat, and fingerless gloves. Normal protocol at OHFs is that the administrators from different companies leave each other alone, but I had to ask. He explained that he was sitting in the only remaining open space in the cage, and it happened to be right underneath an air-conditioning duct that was blasting ice cold air. The air was so cold that he simply had to have his ski parka to be able to work. I couldn t help but wonder what the people from OSHA2 would have said if they had seen these working conditions. Evan

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TABLE 4.2. Selectivity of Immobilization Methods Method Diazotization Triazine Cyanogen bromide Acyl azide Carbodiimide Carbodiimide NHS Anhydride Disul de Amino Acid Residues Modi ed Lys, His, Tyr, Arg, Cys Lys Lys Lys, Tyr, Cys, Ser Lys, Tyr, Cys, Ser, Met Lys Lys Cys

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There was another serious concern that could not have been foreseen in the early, salad days of OHFs. The OHF is in every way a data center for its customers. If the OHF went out of business or closed a facility (which happened a lot near the end, sometimes without any notice), displaced customers were forced to quickly migrate their operations from the closing OHF to a new facility. In another context, a rapid, unforeseen data center migration is called a disaster and could easily result in extended downtime.

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There is no better way to bring home this book s mission statement that achieving true high availability requires more than just installing failover management software than to discuss electricity and electrical dependencies. A system that is functioning perfectly will, of course, stop instantly when its electrical supply is cut off. If two systems are connected together in a highavailability pair, and their electricity is cut off, they will both stop working. By providing electricity through separate paths, their power source, and therefore the systems, becomes more reliable. The farther upstream the power source paths can be separated, the more reliable the power supply gets. Plugging both systems in an HA pair into the same power strip is obviously silly, especially if the power strip has a single on/off switch on it. Running both systems through the same circuit breaker is almost as silly, but much

4.2.2. Cross-Linking with Bifunctional Reagents Cross-linking reagents are used for the covalent immobilization in cases where the enzyme is available in large quantities, and retention of a high percentage of the original activity is not crucial. Cross-linking usually results in a high quantity of immobilized protein, since bonds between enzyme molecules form in addition to enzyme-support bonds. However, loss of activity on immobilization is generally higher if cross-linking occurs, since the polymeric network of enzyme that forms may obscure the active sites, slow the diffusion of substrates and products, and distort the tertiary structure of the enzyme. Cross-linking immobilizations are popular because of their speed and simplicity. Cross-linking reagents consist of two reactive functional groups, separated by a spacer group. The reactive groups may be identical, such as in glutaraldehyde [OHC (CH2)3 CHO], in which case it is called a homobifunctional reagent. If the two functional groups are different, the reagent is a heterobifunctional crosslinker. Common homobifuctional cross-linking reagents are shown in Figure 4.2. They include glutaraldehyde,13 which is selective for primary amines, forming imide linkages that may be subsequently reduced to secondary amines by borohydride reduction; diazobenzidine14 is reactive toward tyrosine residues, but also reacts with histidine nitrogen and lysine; hexamethylene-bis(iodoacetamide),15 which is

The Occupational Health and Safety Administration is the United States government agency that regulates workplace and worker safety.

more common, probably because it s a little harder to see which power outlets are on the same circuit. The extreme at the other end is to obtain power from two different power grids or power companies. While this will provide system protection against some major outages, it can be prohibitively expensive and may introduce problems with grounding or other electrical issues.

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