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selective for lysine amino groups by nucleophilic substitution of iodide; and bis(Nhydroxysuccinimidyl)dithiodipropionate,16 which is not only selective for lysine amino groups, but is also a reversible cross-linking reagent, since the disul de bond may be broken by common reducing agents Heterobifunctional cross-linking reagents are less commonly used, but provide an additional level of selectivity due to the different reactivities of the terminal groups Some commercially available heterobifunctional reagents are shown in Figure 43 Reagents with isocyanate and isothiocyanate groups are selective toward primary amines, with the isocyanate group being reactive at room temperature, and the isothiocyanate reacting at elevated temperatures to form substituted ureas and thioureas, respectively17 Reagents possessing maleimido groups are selective toward thiols, while the NHS ester functionalities react with primary amines18 423 Adsorption Adsorption is the simplest of methods for enzyme immobilization.

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