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3.2. SUBSTRATE DEPLETION OR PRODUCT ACCUMULATION When a substrate can be directly measured, the reason for using an enzymatic assay for its quantitation may not be immediately apparent. However, complex biological media matrices may contain a variety of species that interfere with the direct measurement of analyte concentration. For example, if the analyte absorbs in the visible (vis) or ultraviolet (UV) region, direct quantitation may result in erroneously high values if interfering species absorb at the measurement wavelength. An enzymatic method, on the other hand, can monitor the absorbance decrease that occurs as a result of the selective consumption of analyte by the enzyme, thus avoiding the spectral interference. An assay for uric acid involves the enzyme urate oxidase, which catalyzes the following reaction: ! Uric acid O2 2H2 O Allantoin H2 O2 CO2 3:1 word aflame upci Download UPC-A Font - Free Font Download - Font Palace
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