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of calibration data for substrate and product measurements is necessary for an optimized direct absorbance-based assay. In practice, product accumulation is almost invariably measured in an indirect manner, since it is often possible to couple a second reaction in series with the rst substrate-selective reaction in order to generate an easily monitored secondary product.

JBOD (Just a Bunch of Disks)

8

JBOD (pronounced JAY-bod ) refers to a collection of disks without any sort of hardware intelligence or real organization. Often, JBODs are literally a stack of small disk cabinets that each contain a disk or two. On a critical system, these disks will require some sort of logical volume management and Software RAID capability.

3.3. DIRECT AND COUPLED MEASUREMENTS Some enzymatic reactions can be followed directly, either by substrate depletion or product accumulation measurements, with adequate precision for direct enzymatic assays. However, many enzymes catalyze reactions involving species that are not themselves readily measured. In these situations, products are converted to species that are measurable, in a coupled, or indicator reaction. The indicator reaction may be chemical or enzymatic, and quantitatively converts the product of the primary reaction into a readily measurable species. The main requirement for the indicator reaction, whether it is chemical or enzymatic in nature, is that the conversion of the primary product into the measured product must be rapid and quantitative. ! Substrate Primary product Measured product !

A hot spare is a disk that sits in reserve, waiting for a critical disk to fail. The hot-sparing software agent migrates the data that was on the failed disk to the hot spare. Hot spares require some sort of RAID so that the contents of the failed disk can be re-created on the replacement. Hot sparing can be performed by either the disk array or by logical volume management software.

Many disk arrays contain some specialized memory that acts as a staging area for disk writes. Since memory is much faster than disk, writes to this cache can complete many times faster than writes to disk. Write cache can increase the cost of a disk array, and unless the disk array has a reliable battery backup, it can cause data loss if the data written to the cache never makes it to disk. Data that s written to the write cache is flushed to disk on a regular basis, usually every few seconds.

3:3

Storage Area Network (SAN)

A storage area network is a new model for interconnecting storage and computing systems into a shared pool that many different hosts can access. Although many SANs are based on Fibre Channel, FC is not a requirement. SANs can be based on a number of different underlying technologies, including IP-based networks. We will discuss SANs in more detail in 8. The storage devices on the SAN are usually limited to disks and tape drives, although other devices, such as CD and DVD drives, are expected to be available in the near future. Through the use of protocol bridges, legacy SCSI-based storage devices can join a SAN as well. In Figure 7.5, we offer a diagram of a simple SAN.

An assay for adenosine involves the primary enzyme adenosine deaminase, and a chemical indicator reaction that consumes ammonia by reaction with the ninhydrin reagent (Eq. 3.4):

Discussing application integration without linking it to the problem of IT urbanization would be to miss much of the goal that we have set ourselves in this book. IT urbanization is an approach and a discipline in its own right, whose methodological advances have made possible sustainable development of the information system based on segmentation into business blocks, and the implementation of rules for constructing these blocks (for more information see section 4.2 and the work of Jacques Sassoon [SAS 98]). Our idea here is less to develop the themes behind the urbanization of information systems than to use its concepts and its operational realities for indicating how an approach that links application integration with IT urbanization can multiply benefits and return on investment, making IT more reactive to business developments. 8.1. IT urbanization review The term IT urbanization clearly refers to the range of problems in urban (or city ) planning : each district has its own characteristics (residences, offices, shops, recreation, etc.). An information system can similarly be divided into zones;

Fibre Channel Switches FC to DWDM bridge Tape drives and libraries FC to SCSI bridge Legacy SCSI disk array Remote SAN

7

3:4