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segment written to a separate disk. Because each disk can complete the smaller writes at the same time, write performance is improved over writes to a single disk. Performance can be enhanced further by striping the writes between separate controllers too. The size of each segment is called the stripe width. In general, the optimal size of a stripe width is the average size of a disk write, divided by the number of disks in the stripe. However, if the disk array has its own cache, the rules may change. The first and most important thing to know about RAID-0 is that, unlike all the other levels of RAID, RAID-0 does not increase your system or data availability. In fact, when used by itself, RAID-0 decreases availability. In order for a stripe set to function, all of its member disks must be available. If any disk in a stripe fails, the entire stripe fails and cannot be read or written. Consider a 2GB filesystem, and disks with a 200,000-hour MTBF. If the filesystem lives on a single 2GB disk, the MTBF in is simply 200,000 hours. If the filesystem is striped across four disks, with 500MB of data on each disk, the MTBF is reduced significantly due to the increased probability that at least one of the disks will fail. Because RAID-0 adds no redundancy, it does not require any additional disk space. Please note that when we use the term RAID throughout the book as a generalized way of adding disk redundancy, we are specifically excluding RAID-0 from that discussion, since RAID-0 reduces overall system availability and all other levels increase it.

formation of reduced nicotinamide coenzyme (NADH or NADPH) allows absorbance measurements at 340 nm, where the molar absorptivities4 of the reduced coenzymes are 6:2 103 M 1 cm 1 (Fig. 3.1). Dehydrogenase indicator reactions are common because few interferences exist for absorbance measurements at 340 nm. Peroxidase indicator reactions may be used to follow any primary reaction that produces hydrogen peroxide. Peroxidases are very speci c for H2O2, but will react with a variety of organic dye species that are colorless in the reduced form, but highly absorbing in the oxidized form. Examples include 2,4-dichlorophenol, Odianisidine, malachite green, and benzidine. Two commercial enzymatic assays exist for the determination of glucose in serum. One involves the primary enzyme glucose oxidase and the indicator enzyme peroxidase: Glucose O2 d-D-Gluconolactone H2 O2 ! 3:7 3:8 The oxidized form of O-dianisidine is red, and shows an absorbance maximum at 450 nm with a molar absorptivity5 of 8:6 103 M 1 cm 1 . The second glucose assay involves hexokinase as a primary enzyme, and uses glucose-6-phosphate (G6P) dehydrogenase as an indicator enzyme: Glucose ATP G6P ADP ! G6P NADP

RAID-1, or mirroring, is a model where a copy of every byte on a disk is kept on a second disk. The copy is (normally) 100 percent in sync. In this model, if one disk fails, the other disk continues to operate without interruption. A common misconception of RAID-1 is that a master/slave relationship exists between the disks, and if one disk fails, the other takes over. This is not the case: Both copies in a mirror act as peers. A better way to think about this relationship is to imagine a logical disk that sits on top of the mirrored copies and sends all read and write requests to both disks at the same time. If a disk fails, the logical layer is not affected; it continues reading and writing to the surviving disk. Reads are usually requested of both underlying copies, and the logical layer returns the data that comes from the first physical disk to reply, resulting in a slight improvement in performance under RAID-1. In fact, mirrors are not limited to two copies. Many shops will keep three or four mirrors of their data, and split one off from time to time for backups, or to provide production-like data to their development, quality assurance, or disaster recovery environments.

Another, slightly less common misconception about mirroring is that if your disks are mirrored, you don t need to take backups This is not true Mirrors will protect your users from hardware failure, but they will not protect you from the careless or malicious deletion or corruption of critical data All mirrored copies will be affected by the deletion Point-in-time copies or checkpoints, however, will protect your users from such a deletion; we discuss those later in this chapter Besides the minor performance improvement, the main advantage of RAID-1 is data protection when the inevitable failure of a disk occurs If a disk fails in a RAID-1 configuration, users and applications will continue to receive their data, with no interruption in service RAID-1 has disadvantages as well The primary disadvantage is in the added expense Each mirror requires 100 percent disk overhead It takes an additional 10GB to mirror 10GB.

O-Dianisidine H2 O2 2; 20 -Dimethoxydiphenylquinonediimine 2H2 O !