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As I said in an earlier Tales from the Field, when you write a book like this one, people assume you are an expert on every issue related to the topic, and so you get pulled into all sorts of technical debates and arguments Some are interesting, some are hairsplitting One of the more interesting (and yet hairsplitting) debates was over which name, 0+1 or 1+0, actually referred to mirrored stripes, and which referred to striped mirrors, and for that matter, whether mirrored stripes meant that the mirroring took place first or the striping did The nomenclature that we use in this section was based on some research, as well as on what we see in marketing literature from companies who have products that actually deliver RAID 1+0 and 0+1.

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My worthy opponent in the debate was a colleague, friend, and prolific author, Paul Massiglia, who served on the original RAID Advisory Board, and who had helped name the levels of RAID, including 1+0 and 0+1, back in 1989 The Board had, unfortunately, named them the other way around, and Paul and I were being asked to reach a consensus on the names for VERITAS s marketing department We agreed that the names striped mirrors and mirrored stripes were very confusing, largely because it s hard to remember which is which (and the numbers don t help either) When the board assigned the names, they decided that 0 (striped) + 1 (mirrors) was a sensible naming convention Unfortunately, the convention evolved differently in the marketplace In the end, the argument came down to style rather than any technical issue.

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23 To a lesser degree in the USA and Canada since North American culture has more of a tendency to wipe the slate clean then reconstruct, than to alter the existing system little by little.

The Smith assay takes $ 1 h, and requires a minimum volume of 500 mL with 02 50-mg protein (04 100 mg/mL) As with the Lowry assay, calibration curves are nonlinear, with negative deviation at high protein concentration Interferences include copper reducing agents (such as reducing sugars) and complexing agents as well as acidifying agents in the unknown sample Common membrane lipids and phospholipids have also been shown to interfere, and an apparent protein concentration of 17 mg of protein (as BSA) was found for 100 mg of 1,3-dilinoleoyglycerol4 Since BCA is a stable reagent at alkaline pH, it can be added to the alkaline copper reagent, so that only one reagent addition is required.

We decided to use the style that you see in this book, after a discussion that ran on and off for several months and that was briefly revived (for about the 10th time in the last few years!) as I put this Tale together Evan.

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The difference in layouts between the two models depends on which of the two methods is employed first. As shown in Figure 7.6, in RAID 0+1 (or mirrored stripes), the stripes on one side of the mirror are built first and then they are mirrored to the other side. Figure 7.7 shows RAID 1+0 (or striped mirrors); the mirrors are built separately first, and then are striped together as if they were separate disks.

The Smith assay has been adapted for 96-well microtiter plates,5 and a linear dependence of absorption (570 nm) on protein concentration was observed over the 1 10-mg/mL concentration range, with 10-mL sample volumes 123 Bradford Method The Bradford method is based on the noncovalent binding of the anionic form of the dye Coomassie Blue G-250 with protein6 The dye reacts chie y with arginine residues, which have a positively charged side chain, and slight interactions have also been observed with basic residues (histidine and lysine) and aromatic residues (tyrosine, tryptophan, and phenylalanine) In the absence of protein, the dye reagent is a pale red, and upon binding to protein, a blue color is generated with an absorbance maximum at 590 nm The structure of the dye is shown in Figure 13.

1. Select disks 2. Stripe the disks together 3. Select more disks 2GB 2GB 4. Stripe them together 5. Mirror the sets together 2GB

The differences between 0+1 and 1+0 are not particularly apparent in the description of how they are built. But when you look at how they handle failures and how they recover from those failures, the differences, as well as the advantages of one over the other, become much more striking.

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