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Incremental backups, as we discussed, are a very efficient way to back up file systems, since they require copying far less data to tape then fulls. Data that have not changed since the last full need not be backed up. Unfortunately, cumulative incremental backups can grow in size over time, until their size approaches that of a full. The number of tapes required to restore a set of differential incremental backups can also grow over time until the sheer number becomes overwhelming. The remedy to these problems is the same: Take full backups from time to time. What if you never had to take full backups of your production servers again You can with synthetic full backups. In a synthetic full backup model, you keep a full copy of your filesystem on another node. You take a full backup of your system (curly) once, and restore it on a separate system (shemp). Then, you take nightly differential incremental backups of curly and restore them onto shemp. Then, you take a full backup of the filesystem from shemp. If something happens to curly, you can replace him with shemp. Shemp s data should be identical to what was on curly, and since all the data is on a single tape, the time it takes to complete the restore will be greatly reduced, as compared to restoring a full and a bunch of incrementals.

CHAPTER 11 1 Zone EP causes a continuous migration in one direction, as long as the voltage is applied; bands can migrate right off the gel The IEF causes initial migration, but reaches a steady-state when all proteins are at their pI values and have zero net charge 2 The pH range of the gel is 65 (anode) 75 (cathode), over the 10-cm length The three HB variants will be 08 cm (pI 742), 29 cm (pI 721), and 45 cm (pI 705) from the cathodic end of the gel 3 After the IEF run, the gel is exposed to a solution containing the E Ab conjugate The enzyme is chosen to convert a colorless substrate into a highly absorbing product A colored band appears where the Ab binds its Ag.

For additional protection, you may choose to keep shemp at a different site from curly, although that s not necessary if you send shemp s backup tapes offsite. (No need to keep an on-site copy, since shemp itself fills that role quite admirably.) This model is certainly not for everyone, since it requires a great deal of extra hardware (system, disks, and tape drives), but it is a very effective way to reduce backup windows and restore times, a very elusive combination.

Reaction of proteins with SDS, needed in the second dimension of the 2D separation, results in a loose of the tertiary quaternary structure of the antigen, so that Ab probably will not bind 4 IEF is performed on proteins in their native state, and is based on migration to the pI value in the gel The SDS disturbs the protein structure, and gives a uniformly negative charge/mass ratio, so that separation is based only on size (sieving) 5 Nucleic acids inherently have a uniformly negative ratio charge/mass m=z from the sugar phosphate backbone An IEF run over the pH 6 8 range would cause migration of nucleic acids off the anodic end of the gel 6 The IPG DALT gel shows the presence of two subunits One has pI 82 and MW 50 kDa, while the other has pI 71 and MW 80 kDa.

Vendors are developing new techniques and technologies, and exploiting old ones, to speed up backups through the use of additional hardware or clever software tricks. These techniques are of varying usefulness, depending on your environment. Some will surely work, while others could actually slow you down.

The marketing hype for host-free backups (sometimes called backdoor or direct backups) says that they allow the backing up of data directly from disk to tape, without requiring an intervening CPU The reality of host-free backups is that there must be a CPU someplace There is no technology today that we have found that allows data to simply copy itself to tape There must be a CPU to initiate and perform the copying work Some hardware arrays have embedded CPUs that perform the work Other solutions use dedicated data movers from companies like Chaparral, ADIC, or Crossroads, which contain CPUs that take care of the data copying Other solutions use the host s CPU to copy the data While there may be advantages to using off-host CPUs to copy the data, it is not an absolute given, as many people assume, that the use of host-based CPU is bad.

There has never been a greater need to for integration engines to transform XML formats to other XML formats Why should this be a problem Is this not precisely what integration engines are for This is a problem because these transformations have a high cost in terms of performance (see section 3211) Like variable type formats of which they are only a particular type XML formats require dataflows to be parsed on input to search for the information to be checked or to be transformed in an integration.

Since the 80 kDa band is darker (larger), the intact protein probably contains two 80- and one 50-kDa subunits 7 Molecular weight markers are added at the end of the IEF run, to one end of the IEF gel, so that they do not interfere with (or obscure) the focussing of analyte proteins, and provide a distinct series of bands on one side of the 2D gel..