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10.1. Introduction to the method The deployment of an application integration solution must respond to sometimes contradictory objectives: deploying the solution at the earliest time, to serve the first project that will use it; setting up an exchange infrastructure that is stable enough over time that it does not have to be modified every time a new project wants to use it; producing norms and rules for using the solution in order to maintain overall coherence and adjust the possible incompatibilities between projects; giving each project sufficient autonomy of implementation so that solution use is pertinent in its context; capitalizing on project experience to generalize rules for follow-up projects. The task is not easy. It is therefore particularly important to suggest a method for deploying application integration solutions whose principal objective will be to bring project constraints, infrastructural norms, capitalization and acquired experience into harmonious coexistence. Below, we present the principal lines.

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Nines are an average Maximum outages, in terms of the maximum time to repair, are more important than the average uptime Nines only measure that which can be modeled Load and software are hard to model in an average case; you will need to measure your actual availability and repair intervals for real systems, running real software loads Nines usually reflect a single system view of the world Quick: Think of a system that s not networked but important Reliability has to be based on networks of computers, and the top-to-bottom stack of components that make up the network The most reliable, fault-tolerant system in the world is useless if it sits behind a misconfigured router Computer system vendors talk about nines of availability, and although nines are an interesting way to express availability, they miss some essential points All downtime is not created equal.

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reactors may be used for several months with little change in conversion ef ciencies. Urease immobilized onto a nylon membrane has been studied with respect to its thermal denaturation properties. Figure 4.8 illustrates the comparative thermal stabilities of immobilized and soluble urease, determined by measuring relative activity as a function of incubation time at elevated temperatures.26 Clearly, the immobilized urease exhibits improved thermal stability that results from the protective microenvironment at the surface of the support. A spectacular example of stability enhancement through immobilization has been reported for the enzyme catechol-2,3-dioxygenase.27 This enzyme, isolated from the thermophilic bacterium Bacillus stearothermophilus, catalyzes the conversion of catechol to 2-hydroxymuconic semialdehyde (which can be monitored by absorbance at 375 nm). The soluble enzyme exhibits maximal activity at 50 C, but following immobilization on glyoxyl agarose beads with a borohydride reduction step, the optimum reaction temperature shifted to 70 C. At a total protein concentration of 0.010 mg/mL and a temperature of 55 C, the half-life of the soluble enzyme was 0.08 h, while the enzyme-modi ed beads had a half-life of 68 h. This represents a 750-fold enhancement of stability that has been attributed to the prevention of subunit dissociation upon immobilization. 4.4. IMMOBILIZED ENZYME REACTORS The immobilization of enzymes onto particulate carriers that may be packed into a column (the packed-bed reactor), such as a typical HPLC column, facilitates

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If an outage drives away customers or users, then it is much more costly than an outage that merely inconveniences those users But an outage that causes inconvenience is more costly to an enterprise than an outage that is not detected by users Consider the cost of downtime at a retail e-commerce web site such as amazoncom or ebaycom If, during the course of a year, a single 30-minute outage is suffered, the system has an apparently respectable uptime of 99994 percent If, however, the outage occurs on a Friday evening in early December, it costs a lot more in lost business than the same outage would if it occurred on a Sunday at 4:00 AM local time in July Availability statistics do not make a distinction between the two.

Similarly, if an equities trading firm experiences a 30-minute outage 5 minutes before the Federal Reserve announces a surprise change in interest rates, it would cost the firm considerably more than the same outage would on a Tuesday evening at 8 PM, when no rate change, and indeed, little activity of any kind, was in the offing Consider the frustration level of a customer or user who wants to use a critical system If the 30-minute outage comes all at once, then a user might leave and return later or the next night, and upon returning, stay if everything is OK However, if the 30 minutes of downtime is spread over three consecutive evenings at the same time, users who try to gain access each of those three nights and find systems that are down will be very frustrated Some of them will go elsewhere, never to return.

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