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Some CPU must be used in order to move the data The external CPU that is included in a disk array or data mover may be quite expensive, and it may be completely unnecessary if there are enough idle CPU cycles in the host CPU And most systems have some extra CPU cycles to spare What s more, no matter which CPU does the work, the storage will still be called upon to perform I/Os, and there will likely be some overhead incurred from that work If the copying (backing up) is done from disks that have been split off of the original copies, I/O overhead may still be evident in the SCSI or Fibre Channel connections and/or host bus adapters (HBAs), or over the networks between the clients, hosts, and tape drives Today s technology simply does not support true host-free backups.

In the future, enough intelligence will be placed inside the disk arrays to allow them to push their data directly out to a tape drive, but even then, there is still a CPU.

CHAPTER 12 1. By using Eq. 12.7, retention times (a) increase by a factor of 4, (b) decrease by a factor of 2, and (c) increase by a factor of 2.

6

involved. The advantage that any host-free backup offers is that the backup can be performed without impacting the CPU or memory performance of the host holding the data. However, that CPU may be the cheapest in your system. In many cases it will cost less to get some additional CPU for your host than it would to get additional CPU for your disk array or data mover. It is not automatically evil if a small amount of extra CPU host is consumed. If the backups are also performed over a dedicated backup network or a storage area network (SAN; for more on SANs, see 8, SAN, NAS, and Virtualization ), then little impact should be felt on production resources. EMC Symmetrix arrays have embedded PCs, and they offer off-host backups when combined with certain commercial backup utilities. More and more backup software vendors are adding this capability to their software.

2. The APTES modi cation will cause an anodic drift, so the detector should be placed at the anodic end of the capillary. 3. The lowest m=z corresponds to the highest charge ( 8), and the highest m=z corresponds to the lowest charge ( 5). The four calculated MW values are thus 7504, 7497, and 7500, so MW 7500. 4. All amino acids have the reactive primary amino group, which can be derivatized, for example, with an aldehyde group present on a redox-active species, such as ferrocenecarboxaldehyde, (C5H5)Fe(C5H4CHO). 5. The coincidence detector eliminates background noise by requiring both PMTs to simultaneously record a count. 6. The G:A:C:T ratio is 8:4:2:1. Thus the sequence can be read as 50 -CTGCAGCATCATG-30 . The complementary (analyte) sequence is therefore 50 -CATGATGCTGCAG-30 . 7. To elute the pattern at the anodic (acid) end of the capillary, the focused proteins should develop a negative charge. This is done by shifting to basic conditions by adding a salt (e.g., NaCl) to the anodic buffer reservoir.